Journal: Gene

Article Title: miRNA-1229-5p promotes migration and invasion and suppresses apoptosis of endometrial cells via the STMN1/p38 MAPK axis in endometriosis.

doi: 10.1016/j.gene.2025.149385

Figure Lengend Snippet: Fig. 4. Mir-1229-5p activated p38 mapk signaling pathway. A-B) Western blotting detected the p38 MAPK signaling related protein expression of p38, p-p38, ATF2, p-ATF2, mmp2, mmp9 in transfected ESCs and ISK cells. *P < 0.05, **P < 0.01.

Article Snippet: This was followed by overnight incubation at 4 ◦C with primary antibodies against E-cadherin, N-cadherin, ZEB1, Bcl-2, Bax, mmp2, mmp9, and GAPDH (all sourced from Proteintech, Rosemont, IL, USA), STMN1 (Abcam, USA), p-p38/p38, and p-ATF2/ATF2 (Santa Cruz, USA).

Techniques: Western Blot, Expressing, Transfection

Journal: Gene

Article Title: miRNA-1229-5p promotes migration and invasion and suppresses apoptosis of endometrial cells via the STMN1/p38 MAPK axis in endometriosis.

doi: 10.1016/j.gene.2025.149385

Figure Lengend Snippet: Fig. 7. The re-expression of STMN1 could reversed the p38 MAPK signaling activation of miR-1229-5p overexpression in ESCs and ISK cells. A-B) Western blotting detected the p38 MAPK signaling related protein expression of p38, p-p38, ATF2, p-ATF2, mmp2, mmp9 in transfected ESCs and ISK cells. *P < 0.05, **P < 0.01.

Article Snippet: This was followed by overnight incubation at 4 ◦C with primary antibodies against E-cadherin, N-cadherin, ZEB1, Bcl-2, Bax, mmp2, mmp9, and GAPDH (all sourced from Proteintech, Rosemont, IL, USA), STMN1 (Abcam, USA), p-p38/p38, and p-ATF2/ATF2 (Santa Cruz, USA).

Techniques: Expressing, Activation Assay, Over Expression, Western Blot, Transfection